goat polyclonal anti pink1  (Santa Cruz Biotechnology)

Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.

doi: 10.1038/jcbfm.2008.167

Figure Lengend Snippet: Figure 1 Representative Western blot for DJ-1, PINK1, and a-Syn. Transient ischemia affects expression profile of DJ-1, PINK1, and a-Syn at 8 h of reperfusion. 20 mg of protein from each samples were run on the gel for 90 mins at 20 mA. The proteins on the gel were then transferred to a PVDF membrane, they were then incubated with primary antibodies at 1:1000 dilution for 20 h at 41C. The blots were developed using Chromogenic detection method.

Article Snippet: The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).

Techniques: Western Blot, Expressing, Membrane, Incubation

Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.

doi: 10.1038/jcbfm.2008.167

Figure Lengend Snippet: Figure 2 Immunostaining against DJ-1, PINK1, and a-Syn in motor neurons in a sham spinal cord (A, D, and G); at 8 h (B, E, and H), 1 day (C, F, and I) of reperfusion. Spinal cord sections were incubated with primary antibodies for 20 h at 41C, respectively. The primary antibodies (1:200 dilution, respectively) used were the same as those used for Western blot analysis noted above. The slices were colorized with DAB/H2O2 solution. Bar = 100 mm.

Article Snippet: The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).

Techniques: Immunostaining, Incubation, Western Blot

Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.

doi: 10.1038/jcbfm.2008.167

Figure Lengend Snippet: Figure 3 Co-localization of DJ-1 and PINK1 (A–C), a-Syn and PINK1 in motor neurons (D–F) at 8 h after ischemia. Then, the sections were incubated with DJ-1 antibodies 1:100 simultaneously with PINK1, and the sections were incubated with a-Syn antibodies 1:100 simultaneously with PINK1. The sections were detected by using donkey anti-mouse lgG linked with fluorescein isothiocyanate 1:50 and donkey anti-goat lgG linked with Texas Red I:50 and observed using fluorescein microscopy. Bar = 50 mm.

Article Snippet: The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).

Techniques: Incubation, Microscopy